Maximal oxygen consumption measurements nominally incorporate the use of a mixing chamber (AFT15A/B), facemask with non-rebreathing T valve (AFT25) and an air flow transducer (TSD107B). Typically, air flow measurements are performed on the inspiration side of the AFT25. The expiration side is directed to the AFT15, where O2 and CO2 concentrations are monitored using the O2100C and CO2100C, respectively. Use the Expression Transformation in AcqKnowledge® to perform the Haldane transformation and STP corrections. The final result provides real-time oxygen consumption measurements, permitting precise determination of VO2 maximum, deficit, and post-exercise consumption.