This BSL PRO lesson describes the hardware and software setup necessary to record Compound Action Potentials (CAP) from a dissected frog sciatic nerve. For a specific procedure on isolating and removing the frog sciatic nerve, please refer to PRO Lesson A01 Frog Preparation.
To record the CAP of the frog sciatic nerve and measure the latent period.
To record the effect, on the amplitude of the CAP, of subthreshold, threshold, submaximal, maximal, and supramaximal stimulation of the sciatic nerve.
To record the effects of temperature on the velocity of nerve impulse conduction.
To record the effect on the ability of the nerve to conduct impulses before and after the application of a local anaesthetic.
* To estimate the refractory period of the nerve.
* To explore the relationship between stimulus strength and duration.
*To determine average conduction velocity.
*These objectives are covered in the introductory text and recording template for BSL 4.0.1 or above only.
Tasks Performed by the Student
Optional: Use string as an experimental control to record and examine Stimulus Artifact.
Stimulate the nerve at subthreshold, threshold, submaximal, maximal, and supramaximal levels.
Irrigate the nerve with warmed Ringer’s and then stimulate until Threshold is observed.
Irrigate the nerve with cold Ringer’s and then stimulate until Threshold is observed.
Saturate the nerve with a nerve blocker and then stimulate until Threshold is observed.
Detailed Lesson Procedure (.pdf) and graph template file (.gtl) ensure consistent setup for subsequent Subjects or repeated trials.
BSL 4.0.1 and above: expanded lesson with comprehensive introductory text and updated template
Scripted template guides students with prompts & buttons (similar to standard BSL Lessons)
Simplified setup – template detects hardware and adjusts calibration and scaling
Displays only menus and/or measurements useful for each specific experiment segment
Automatically inserts and labels event markers for experiment segments
Easy to rewind, redo, skip, or add experimental segments
BSL 3.6.6-4.00: original lesson and template; current release for -ES, -IT, -JP, and -RU translations
Add event markers throughout the recording.
The software automates the calibration procedure—there are no knobs and dials to confuse the student.
Graph template files ensure consistent setup for subsequent Subjects or repeated trials—plus, you can easily add your own lesson plan to the onscreen journal.
The lesson will also work as part of a group study.
Student Prep & Distance Learning
Click the link(s) below for sample data and/or lesson procedure video(s), BSL PRO Lesson procedures (PDF) for human lessons*, and graph template files (*.gtl) for BSL PRO Lessons. If more than one .gtl is available, download the .gtl with the _suffix to match BSL version and hardware.
This lesson requires a Biopac Student Lab (BSL) System and the following hardware. If your BSL System does not include all hardware items, expand your system by selecting required items below. For more details, review the Lesson: L# BSL Lessons - see the Lab Manual or launch BSL; A# and H# BSL PRO Lessons, click the PDF link above to review full setup, recording, and analysis procedures.
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