BIOPAC® Systems, Inc. Logo

A03 Frog Sciatic Nerve

a03data.gif
This BSL PRO lesson describes the hardware and software setup necessary to record Compound Action Potentials (CAP) from a dissected frog sciatic nerve. For a specific procedure on isolating and removing the frog sciatic nerve, please refer to PRO Lesson A01 Frog Preparation.

Experimental Objectives

  1. To record the CAP of the frog sciatic nerve and measure the latent period.
  2. To record the effect, on the amplitude of the CAP, of subthreshold, threshold, submaximal, maximal, and supramaximal stimulation of the sciatic nerve.
  3. To record the effects of temperature on the velocity of nerve impulse conduction.
  4. To record the effect on the ability of the nerve to conduct impulses before and after the application of a local anaesthetic.
  5. * To estimate the refractory period of the nerve.
  6. * To explore the relationship between stimulus strength and duration.
  7. * To determine average conduction velocity.

* These objectives are covered in the introductory text and recording template for BSL 4.0.1 or above only.

Tasks Performed by the Student

  1. Optional: Use string as an experimental control to record and examine Stimulus Artifact.
  2. Stimulate the nerve at subthreshold, threshold, submaximal, maximal, and supramaximal levels.
  3. Irrigate the nerve with warmed Ringer’s and then stimulate until Threshold is observed.
  4. Irrigate the nerve with cold Ringer’s and then stimulate until Threshold is observed.
  5. Saturate the nerve with a nerve blocker and then stimulate until Threshold is observed.

Student Prep & Distance Learning

Click the link(s) below for sample data and/or lesson procedure video(s), BSL PRO Lesson procedures (PDF) for human lessons*, and graph template files (*.gtl) for BSL PRO Lessons. If more than one .gtl is available, download the .gtl with the _suffix to match BSL version and hardware.

Lesson Hardware

This lesson requires a Biopac Student Lab (BSL) System and the following hardware. If your BSL System does not include all hardware items, expand your system by selecting required items below. For more details, review the Lesson: L# BSL Lessons - see the Lab Manual or launch BSL; A# and H# BSL PRO Lessons, click the PDF link above to review full setup, recording, and analysis procedures.

Item Name Cart
NERVE1 Nerve Cond Chamber w/drain+lid Add to Cart
ELPAD Abrasive Pads 10/pk Add to Cart
BSLCBL2A Stim - BSLSTM to 2 mm ban Add to Cart
BSLCBL4B Record, 3-lead w/2-mm banana Add to Cart
OUT3 BNC adapter for MP36 built-in stim Add to Cart
Spotlight On
Small Animal SpO2 in MRI

Small Animal SpO2 in MRI

Complete system for small animal SpO2 measurements in an MRI Small Animal Noninvasive Vital Signs Monitor Works on conscious or anesthetized subjects Patented sensor supports heart rates in the range of 90-900 BPM Works with neonatal mice up to 500 gram rats MRI sensor works in small, large and closed bore MRI environments up to 19T […]

View All
Latest News

BIOPAC Ranked #1 for Physiology Experiments by HAPS Members

The Human Anatomy & Physiology Society (HAPS) found that BIOPAC’s Student Lab System (BSL) and AcqKnowledge software were the number one ranked data acquisition and analysis systems for physiology experiments performed in introductory A&P labs. According to HAPS surveys conducted in 2013 and 2016, BIOPAC products are used and preferred by members in more labs than […]

Integrating Eye Tracking Data with Neuromarketing Parameters

Hardware and software packages from BIOPAC allow commercial consumer marketing teams to easily combine precise eye tracking data with physiological data recording. Here are a few notable studies recording physiology with eye tracking data: Predicting Advertising Success: While neurophysiological methods have become increasingly popular to better understand marketing phenomena among academics and practitioners, the success of […]

Read All
Request a Demonstration
Request a Demonstration