BIOPAC® Systems, Inc. Logo

A03 Frog Sciatic Nerve

View Cart “BNC adapter for MP36 built-in stim” has been added to your cart.
This BSL PRO lesson describes the hardware and software setup necessary to record Compound Action Potentials (CAP) from a dissected frog sciatic nerve. For a specific procedure on isolating and removing the frog sciatic nerve, please refer to PRO Lesson A01 Frog Preparation.

Experimental Objectives

  1. To record the CAP of the frog sciatic nerve and measure the latent period.
  2. To record the effect, on the amplitude of the CAP, of subthreshold, threshold, submaximal, maximal, and supramaximal stimulation of the sciatic nerve.
  3. To record the effects of temperature on the velocity of nerve impulse conduction.
  4. To record the effect on the ability of the nerve to conduct impulses before and after the application of a local anaesthetic.
  5. * To estimate the refractory period of the nerve.
  6. * To explore the relationship between stimulus strength and duration.
  7. * To determine average conduction velocity.

* These objectives are covered in the introductory text and recording template for BSL 4.0.1 or above only.

Tasks Performed by the Student

  1. Optional: Use string as an experimental control to record and examine Stimulus Artifact.
  2. Stimulate the nerve at subthreshold, threshold, submaximal, maximal, and supramaximal levels.
  3. Irrigate the nerve with warmed Ringer’s and then stimulate until Threshold is observed.
  4. Irrigate the nerve with cold Ringer’s and then stimulate until Threshold is observed.
  5. Saturate the nerve with a nerve blocker and then stimulate until Threshold is observed.

Videos

Biopac Student Lab Student Download

Student Prep & Distance Learning

Click the link(s) below for sample data and/or lesson procedure video(s), BSL PRO Lesson procedures (PDF) for human lessons*, and graph template files (*.gtl) for BSL PRO Lessons. If more than one .gtl is available, download the .gtl with the _suffix to match BSL version and hardware.

Lesson Hardware

This lesson requires a Biopac Student Lab (BSL) System and the following hardware. If your BSL System does not include all hardware items, expand your system by selecting required items below. For more details, review the Lesson: L# BSL Lessons - see the Lab Manual or launch BSL; A# and H# BSL PRO Lessons, click the PDF link above to review full setup, recording, and analysis procedures.

Item Name Cart
NERVE1 Nerve Cond Chamber w/drain+lid Add to Cart
ELPAD Abrasive Pads 10/pk Add to Cart
BSLCBL2A Stim - BSLSTM to 2 mm ban Add to Cart
BSLCBL4B Record, 3-lead w/2-mm banana Add to Cart
OUT3 BNC adapter for MP36 built-in stim Add to Cart
Spotlight On
Smart Center controller, 2-3 wireless transmitters & AcqKnowledge plus case

Smart Center | Stand-alone BioNomadix Wireless Systems

Smart Center Stand-alone, Portable Wireless Physiology Systems Smart Center Essentials entry-level system includes a Smart Center Device (BN-SMART with USB power cable), 2 or 3 Transmitters (BN-xxx-T with chargers); AcqKnowledge for Smart Center, and a case. Smart Center Enhanced adds a Logger (BN-LOGGER) and Basic Scripting License (ACK100W-BAS) to the Smart Center Device (BN-SMART with powercable), 3 […]

View All
Latest News

New Citations | Neuroscience Studies with BIOPAC

Effects of MDMA on attention to positive social cues for behavioral disorders The psychostimulant drug 3,4-methylenedioxymethamphetamine (MDMA) reportedly produces distinctive feelings of empathy, closeness with others and increased social behavior. Researchers are exploring the potential of this chemical for treatment of psychiatric disorders, such as autism spectrum disorder (ASD) and post-traumatic stress disorder (PTSD). This […]

Electrocardiography Guide Now Available

BIOPAC’s just released Introductory ECG Guide addresses fundamental to advanced concerns to optimize electrocardiography data recording and analysis. Topics include: ECG Complex; Electrical and Mechanical Sequence of a Heartbeat; Systole and Diastole; Configurations for Lead I, Lead II, Lead III, 6-lead ECG, 12-lead ECG, precordial leads; Ventricular Late Potentials (VLPs); ECG Measurement Tools; Automated Analysis Routines […]

Read All
Request a Demonstration
Request a Demonstration