This BSL PRO Lesson explains how to isolate the gastrocnemius muscle of the frog and its somatic motor nerve (the sciatic nerve) and describes the hardware and software setup necessary to record threshold voltage and contractile responses.
To record the twitch threshold, maximal twitch response, summation, tetanus and fatigue for frog skeletal muscle and its motor nerve.
* Examine the effects of the length of the sarcomere on the force of contraction.
* Observe how the pH of the muscle changes as it becomes fatigued.
* These objectives are covered in the introductory text and recording template for BSL 4.0.1 or above only.
Tasks Performed by the Student
Isolate the gastrocnemius muscle and stimulate to observe and measure threshold and maximal response, and summation, tetanus, and fatigue.
Detailed Lesson Procedure (.pdf) and graph template file (.gtl) ensure consistent setup for subsequent Subjects or repeated trials.
BSL 4.0.1 and above: expanded lesson with comprehensive introductory text and updated template
Scripted template guides students with prompts & buttons (similar to standard BSL Lessons)
Simplified setup – template detects hardware and adjusts calibration and scaling
Displays only menus and/or measurements useful for each specific experiment segment
Automatically inserts and labels event markers for experiment segments
Easy to rewind, redo, skip, or add experimental segments
BSL 3.6.6-4.00: original lesson and template; current release for -ES, -IT, -JP, and -RU translations
The MP36 gtl settings use an acquisition rate of 2,000 samples per second in order to visualize the 0.5 msec. pulse width and SCALE Value 2 (voltage setting for the stimulator) is 10 Volts.
Add event markers throughout the recording.
The software automates the calibration procedure for the student—no knobs and dials to confuse the student.
Easily add your own lesson plan to the onscreen journal.
The lesson will also work as part of a group study.
Student Prep & Distance Learning
Click the link(s) below for sample data and/or lesson procedure video(s), BSL PRO Lesson procedures (PDF) for human lessons*, and graph template files (*.gtl) for BSL PRO Lessons. If more than one .gtl is available, download the .gtl with the _suffix to match BSL version and hardware.
This lesson requires a Biopac Student Lab (BSL) System and the following hardware. If your BSL System does not include all hardware items, expand your system by selecting required items below. For more details, review the Lesson: L# BSL Lessons - see the Lab Manual or launch BSL; A# and H# BSL PRO Lessons, click the PDF link above to review full setup, recording, and analysis procedures.
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