BIOPAC® Systems, Inc. Logo

A07 Fish Respiration & Q10

a07data.gif

In this lesson, the Q10 principle is demonstrated by measuring the metabolic rate of goldfish at two different temperatures: 22° C (acclimation temperature) and 32° C (acute exposure temperature). The fish are placed in a sealed metabolic chamber with a known amount of oxygen that will decline as the fish consumes it over time (~30 min). Temperature of the water in the chamber is controlled using a water bath. Changes in oxygen levels are recorded using the BIOPAC MP30 System for later data analysis.

Experimental Objectives

  1. Demonstrate the Q10 principle by measuring the metabolic rate of goldfish at two different temperatures: 22° C (acclimation temperature) and 32° C (acute exposure temperature).
    • Monitor the decline in a known amount of oxygen as the fish consumes it over time (~30 min.) in a sealed chamber of water at each temperature, using the BIOPAC Dissolved Oxygen Probe Transducer (SS69L).
    • Record the change using the BIOPAC MP30 System.
    • Measure the change in the partial pressure of oxygen (PO2) and use the solubility coefficient to calculate the change in content.
  2. Calculate the oxygen consumption of the fish at the two different temperatures.
  3. Calculate the Q10 of fish acclimated to 22° C.

Tasks Performed by the Student

Videos

Biopac Student Lab Student Download

Student Prep & Distance Learning

Click the link(s) below for sample data and/or lesson procedure video(s), BSL PRO Lesson procedures (PDF) for human lessons*, and graph template files (*.gtl) for BSL PRO Lessons. If more than one .gtl is available, download the .gtl with the _suffix to match BSL version and hardware.

Lesson Hardware

This lesson requires a Biopac Student Lab (BSL) System and the following hardware. If your BSL System does not include all hardware items, expand your system by selecting required items below. For more details, review the Lesson: L# BSL Lessons - see the Lab Manual or launch BSL; A# and H# BSL PRO Lessons, click the PDF link above to review full setup, recording, and analysis procedures.

Item Name Cart
SS69L Dissolved O2 Probe - MP3X/45 Add to Cart
SS8L Temp., Liquid immersion, BSL Add to Cart
Spotlight On
short, lightweight pinch leads

LEAD115 Lighter Lead Series

New series of unshielded 45 cm electrode leads with thin cable; suitable for facial EMG and other areas where lighter, shorter lead cables are required. Use for female Touchproof connectors to pinch clip connectors—connect electrodes to either a C-series amplifier or an MEC. The pinch connectors are light weight and the lead cable tinsel wire […]

View All
Latest News

MEAP and BIOPAC used to quantify rapid changes in cardiovascular state

The new issue of Psychophysiology has a study “Quantifying rapid changes in cardiovascular state with a moving ensemble average” using MEAP for preprocessing/analysis and BIOPAC hardware and software for data collection, display, and storage. The proof of concept study demonstrates a viable method for the adoption of ICG measures across the field of psychophysiology. MEAP (moving ensemble […]

News Citations—fMRI & Temperature, Postpartum Depression, and Fibromyalgia

BIOPAC provides software and hardware that allow for research teams to record and analyze respiration activity in physiological experimentation. Here are a few notable studies covering MRI, ECG, and Laser Doppler Flow measurement. fMRI & Thermal Perception: The neural mechanisms underlying thermal perception (how hot or cold we perceive the temperature to be) have not been fully […]

Read All
Request a Demonstration
Request a Demonstration