This BSL PRO Lesson explains how to prepare the frog heart and describes the hardware and software setup necessary to record cardiac rate and contractile responses of the frog heart.
To observe and record atrial and ventricular behavior (systole and diastole) in the frog heart.
To observe and record the cardiac phenomena of refraction, ventricular extrasystole, and the compensatory pause in the frog heart.
To observe and record the effect of increasing and decreasing the temperature of cardiac muscle on cardiac rate and contractility.
To observe and record the effects of acetylcholine, atropine, pilocarpine, and epinephrine on the frequency and amplitude of cardiac muscle contraction in the frog.
To observe and record the positive inotropic effect of digitalis on the frog heart.
Optional: To observe the property of myogenicity in the excised frog heart.
Optional: To observe and record the effects of atrioventricular block induced by Stannius ligature in the frog heart.
Tasks Performed by the Student
Prepare the frog for recording from the heart
Test the frog heart’s reaction to hot, cold, acetylcholine atropine, pilocarpine, epinephrine, and digitalis
Optional: Tie a Stannius ligature and/or excise the frog heart
Detailed Lesson Procedure (.pdf) and graph template file (.gtl) ensure consistent setup for subsequent Subjects or repeated trials.
BSL 4.0.1 and above: expanded lesson with comprehensive introductory text and updated template
Scripted template guides students with prompts & buttons (similar to standard BSL Lessons)
Simplified setup – template detects hardware and adjusts calibration and scaling
Displays only menus and/or measurements useful for each specific experiment segment
Automatically inserts and labels event markers for experiment segments
Easy to rewind, redo, skip, or add experimental segments
BSL 3.6.6-4.00: original lesson and template; current release for -ES, -IT, -JP, and -RU translations
Add event markers throughout the recording.
The software automates the calibration procedure for the student. There are no knobs and dials to confuse the student.
Graph template files ensure consistent setup for subsequent Subjects or repeated trials—plus, you can easily add your own lesson plan to the onscreen journal.
The lesson will also work as part of a group study.
Student Prep & Distance Learning
Click the link(s) below for sample data and/or lesson procedure video(s), BSL PRO Lesson procedures (PDF) for human lessons*, and graph template files (*.gtl) for BSL PRO Lessons. If more than one .gtl is available, download the .gtl with the _suffix to match BSL version and hardware.
This lesson requires a Biopac Student Lab (BSL) System and the following hardware. If your BSL System does not include all hardware items, expand your system by selecting required items below. For more details, review the Lesson: L# BSL Lessons - see the Lab Manual or launch BSL; A# and H# BSL PRO Lessons, click the PDF link above to review full setup, recording, and analysis procedures.
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