This BSL PRO lesson examines and records action potential from cardiac tissue in an amphibian. The lesson procedure and template were developed for BSL 4.0.1 or above.
To make an intracellular electrical recording in cardiac tissue.
To observe and understand the electrical properties of myocardial cells in the vertebrate heart.
To develop an understanding of the ionic basis of the electrical signals in cardiac tissue.
To study the effects of pharmacological probes on the cardiac action potential.
Tasks Performed by the Student
Detect the action potential signal and record cardiac potentials from several regions of an isolated heart.
Apply Epinephrine (EPI), Acetylcholine (ACH), Nifedipine (NIF), and optionally, Na+ free or 10 mM K+ Frog Ringer’s to understand how heart function is modulated.
Adjust the perfusion system flow control as needed.
Position the glass electrode with the aid of a microscope and micromanipulator.
Start and stop the recording, place event markers, monitor the data, and provide feedback.
Comprehensive introductory physiology text.
Easily place and label event/segment markers throughout the recording.
The software automates the calibration procedure for the student. There are no knobs and dials to confuse the student.
Graph template files ensure consistent setup for subsequent or repeated trials—plus, it’s easy to add your own lesson plan to the onscreen journal.
The lesson will also work as part of a group study.
Student Prep & Distance Learning
Click the link(s) below for sample data and/or lesson procedure video(s), BSL PRO Lesson procedures (PDF) for human lessons*, and graph template files (*.gtl) for BSL PRO Lessons. If more than one .gtl is available, download the .gtl with the _suffix to match BSL version and hardware.
This lesson requires a Biopac Student Lab (BSL) System and the following hardware. If your BSL System does not include all hardware items, expand your system by selecting required items below. For more details, review the Lesson: L# BSL Lessons - see the Lab Manual or launch BSL; A# and H# BSL PRO Lessons, click the PDF link above to review full setup, recording, and analysis procedures.
Simple and noninvasive—finger sensor provides accurate & immediate feedback: arterial BP, cardiac output, fluid and hemodynamic status The NIBP100D-HD noninvasive blood pressure system provides a continuous, beat-to-beat, blood pressure signal recorded from the fingers of a subject. Measure noninvasive BP parameters (BP, sBP, dBP, mBP, and PR) plus hemodynamic parameters (PPV, SVV, CO, CI, SV,SI, SVR, […]
The Human Anatomy & Physiology Society (HAPS) found that BIOPAC’s Student Lab System (BSL) and AcqKnowledge software were the number one ranked data acquisition and analysis systems for physiology experiments performed in introductory A&P labs. According to HAPS surveys conducted in 2013 and 2016, BIOPAC products are used and preferred by members in more labs than […]
Hardware and software packages from BIOPAC allow commercial consumer marketing teams to easily combine precise eye tracking data with physiological data recording. Here are a few notable studies recording physiology with eye tracking data: Predicting Advertising Success: While neurophysiological methods have become increasingly popular to better understand marketing phenomena among academics and practitioners, the success of […]