AcqKnowledge Software

The Premier Data Acquisition & Analysis Program

The most trusted & flexible platform for life science research

The preferred application for better science and greater discovery

AcqKnowledge Software - Win
Part #: ACK100W, ACK100M

The Power of AcqKnowledge

AcqKnowledge is used by thousands of labs around the world and has been cited in thousands of peer reviewed publications. AcqKnowledge is included with each MP160, MP36R, and B-Alert system—it can also be purchased separately with this part number.

AcqKnowledge is an interactive, intuitive program that lets you instantly view, measure, transform, replay, and analyze data.

Perform complex data acquisition, stimulation, triggering and analyses using simple pull-down menus and dialogs—no need to learn a programming language or new protocol.

Online analysis settings, filters, and transformations provide real-time feedback, or you can choose from a wide variety of off-line analysis tools. Multiple display options are available during and after acquisition—just click on an icon to flip between Chart, Scope, X/Y, Overlapped segments, Histogram, or FFT.

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Watch the Video Overview of some of the newest User Interface Features!

Fully Customizable Recording!

Configure detailed recording protocols with analog, digital, and calculation channels. Easily select and set up your recording channels simply by choosing your amplifiers from the module list. Select from dozens of preconfigured preset calculation channels such as heart rate, respiration rate, flow, volume, dP/dt, and more! Plus, create additional presets and save them for further use.

AcqKnowledge has user-selectable acquisition modes (including averaging), sample rates, durations, and automation. Graph windows are also completely customizable allowing multiple views of the same data. Rearrange toolbars, add custom buttons for analysis routines or transformations, change the color or size of waveforms, grids, backgrounds, text, and more!

With AcqKnowledge, record from multiple hardware devices at the same time! Record from multiple MP150’s, an MP150 and MP36R, B-Alert and more!

Windows Only – Take advantage of the integrated Media functionality to synchronize your data file to a video recording from a webcam or other camera system. The video and data file will be locked together for playback, allowing the user to simply scroll through either the video or data file and be taken to that same point linked in the corresponding file.

Media Menu

During recording, select from dozens of available preset channels both for amplifiers and calculation channels. Spend less time on set-up, and get from recording to reporting using AcqKnowledge’s powerful suite of analysis routines.

Use the scaling tools to customize the divisions and scaling of both the horizontal and vertical axis. Fully customize the divisions, color, and scaling of the channel grids and save presets for the grid settings used most often (ex: ECG grid).

Working with hardware or software from another manufacturer? No problem, AcqKnowledge has import and export features that work with a wide variety of data formats including MatLab, Text, Excel, Noldus Observer XT, and SMI BeGaze.

Stimulation—AcqKnowledge can output a range of stimulus paradigms for constant current and constant voltage stimulation. It accepts digital events from virtual reality environments and stimulus presentation systems such as E-Prime, Presentation and SuperLab and categorizes them for further analysis. Easily configure single pulses, arbitrary waveforms, and pulse chains for stimulation!

Targeted Analysis

Take advantage of fully automated analysis routines created to save you time. For ECG data utilize the automated HRV analysis, or locate and mark ECG complex boundaries for both human and animal signals. Take advantage of multiple routines available for EEG, EDA, EMG, and Respiration. Look at RSA or perform a detailed ICG analysis with a few clicks of the mouse. Routines are available for most physiological signals! Further target analysis by creating Focus Areas. Select areas of interest within the data file, and mark them as focus areas. Then, simply choose an analysis routine and run it on the Focus Areas only, as opposed to the whole file!

Watch AcqKnowledge Tutorial Screencasts to learn more now.Watch Now

Analysis RoutinesAcqKnowledge also has a wide variety of simple transformations and filters available for use as well. Filter both during recording or after, find Rates, Cycles, and take multiple measurements over selected data areas. Perform FFTs, and other spectral analyses over selected areas of data or the whole file. Create and evaluate expressions, use the detailed find cycle tool and export results to the journal, as an Excel spreadsheet, 3D plot, and more!

Industry Leading Support
Have a question about the software, or an analysis routine? BIOPAC provides a wide variety of support tools at your fingertips! A detailed software guide, dozens of software video tutorials, application notes, and the knowledge base provide instant answers. If more information is needed, BIOPAC’s technical support team can provide help by phone or email.

Newest features in AcqKnowledge include…

  • Automatic fixed delay option for NIBP100D when BioNomadix is being used
  • Easily open scripts from within AcqKnowledge
  • Arbitrary wave stimulation for MP36R units (output an arbitrary wave similar to the arbitrary wave output for MP150 stimulator)
  • Access About panel from the Startup Wizard
  • Determine file version in scripting
  • Extract file details from the OS
  • Disable closing of Check Impedance Control Panel while the check impedance is in progress.
  • Sweep display mode during acquisition added
  • Toggle visibility of individual PV loops and of fitted slopes in Pressure-Volume Loop analysis


Integrating BIOPAC Research Systems | Data Acquisition & Analysis


Application Notes



Knowledge Base



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Unmatched Power & Flexibility

Automated analysis for ECG, HRV, EEG, EMG, EGG, and many more. Compatible with major manufacturers’ data formats.

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Intuitive, Elegant Interface

Enhanced user interface and menus simplify operation. New Measurement Result Mouse-Overs. Quick-Start Templates.

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Designed for Discovery

Applicable to over 40 academic research fields, plus neuromarketing & commercial applications.

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