BIOPAC data acquisition and analysis for education
Data acquisition and analysis systems for the life sciences BIOPAC life science data acquisition and analysis systems, stimulators, transducers, and more...

BIOPAC video tutorials for life science research and teaching systems

Overview of data acquisition and analysis systems for research

Biopac Student Lab overview

New! Biopac Science Lab for secondary education

Product releases

Announcements & Product Updates


Stay Connected
  Applications    Exercise Physiology  Advanced Features   
Request Information

HRV analysis AcqKnowledge 

AcqKnowledge® software includes a fully-automated Heart Rate Variability analysis tool. The algorithm conforms to the frequency domain algorithm guidelines as published by the European Heart Journal. For optimal results, follow these suggestions for Preparing HRV Data for Analysis.

  • There are two methods for extracting the RR intervals from the ECG signal:

    • A modified Pan-Tompkins QRS detector
    • Events located in the file - ECG QRS Peak event

ECG Events for HRV Analysis

  • The RR intervals are re-sampled to a continuous sampling rate in order to extract frequency information.

    • Cubic-spline interpolation is used to generate this continuous time-domain representation of the RR intervals.

HRV Interpolated Tachogram

  • The frequency information is extracted from the RR intervals and analyzed to produce standard ratios. Power sums are reported in units of sec2/Hz. A Welch periodogram is used to generate the Power Spectral Density (equivalent to Transform > Power Spectral Density).

 HRV Analysis PSD data

  • The software reports values for the typical HRV frequency bands, including: Very Low Frequency, Low Frequency, High Frequency, and Very High Frequency ranges. The analysis also includes values for the overall ratios: Sympathetic, Vagal and Sympathetic - Vagal balance. The ratio values and frequency band results can be plotted into a Journal file or exported for further analysis.

AcqKnowledge also inlcudes a Poincare plot option that will display either selected areas of the data, or the entire file. The Poincare plot is an X/Y plot of the RR intervals in seconds. One axis displays the RR interval and the other axis displays the interval delayed by one beat.

HRV Analysis Poincare Plot

Note: The BioHarness is not recommended for heart rate variability studies because the sample rate used to record the ECG waveform is at the very low end of the optimal range for HRV studies.

Advanced Feature Hardware

Item Description
ACK100M AcqKnowledge Software - Mac
ACK100M-G AcqKnowledge Software GLP - Mac
ACK100W AcqKnowledge Software - Win
ACK100W-G AcqKnowledge Software GLP - Win
ECG100C ECG Electrocardiogram Amplifier
MEC110C C-series Biop. Amp to Touchproof
TEL100C 4-ch Remote Monitoring Telemetry Sys
Click here to find more information on Research Click here for more information on Education