This PRO lesson describes the hardware and software setup necessary to record Compound Action Potentials (CAP) from a dissected frog sciatic nerve. For a specific procedure on isolating and removing the frog sciatic nerve, please refer to PRO Lesson A01 Frog Preparation.
EXPERIMENTAL OBJECTIVES
To record the CAP of the frog sciatic nerve and measure the latent period.
To record the effect, on the amplitude of the CAP, of subthreshold, threshold, submaximal, maximal, and supramaximal stimulation of the sciatic nerve.
To determine the average nerve conduction velocity.
To observe and record nerve refractoriness by stimulating the nerve using maximal stimuli of increasing frequency.
To record the effects of temperature on the velocity of nerve impulse conduction.
To record the effect on the ability of the nerve to conduct impulses before and after the application of a local anaesthetic.
TASKS PERFORMED BY THE STUDENT
KEY FEATURES
Add event markers throughout the recording.
The software automates the calibration procedure for the student. There are no knobs and dials to confuse the student.
Graph template files ensure consistent setup for subsequent Subjects or repeated trials—plus, you can easily add your own lesson plan to the onscreen journal.
The lesson will also work as part of a group study.
)
